Description
p53 Antibody / TP53 | V9400-100UG | Gentaur US, UK & Europe Disrtribition
Family: Primary antibody
Formulation: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Format: Purified
Clone: rTP53/6927
Host: Mouse
Clonality: Recombinant Mouse Monoclonal
Isotype: Mouse IgG2a, kappa
Species Reactivity: Human
Application: IHC-P
Application Details: Immunohistochemistry (FFPE): 1-2ug/ml
Application Note: Optimal dilution of the recombinant TP53 antibody should be determined by the researcher.
Purity: Protein A/G affinity
Description: Recognizes a 53kDa protein, which is identified as p53 suppressor gene product. It reacts with the mutant as well as the wild form of p53 protein. p53 is a tumor suppressor gene expressed in a wide variety of tissue types and is involved in regulating cell growth, replication, and apoptosis. It binds to MDM2, SV40 T antigen and human papilloma virus E6 protein. Positive nuclear staining with p53 antibody has been reported to be a negative prognostic factor in breast carcinoma, lung carcinoma, colorectal, and urothelial carcinoma. Anti-p53 positivity has also been used to differentiate uterine serous carcinoma from endometrioid carcinoma as well as to detect intratubular germ cell neoplasia. Mutations involving p53 are found in a wide variety of malignant tumors, including breast, ovarian, bladder, colon, lung, and melanoma.
Immunogen: Recombinant full-length human TP53 protein was used as the immunogen for the recombinant TP53 antibody.
Storage: Aliquot the recombinant TP53 antibody and store frozen at -20 °C or colder. Avoid repeated freeze-thaw cycles.
Localization: Nucleus
