P3X63Ag8 cell line | CL-0181

P3X63Ag8 cell line | CL-0181 | Gentaur US, UK & Europe Disrtribition

Media: CM-0181

Organism: Mus musculus, Mouse

Application: N/A

Background: This cell ine is derived from the P3K27 cell line (a tissue culture line from the MOPC-21 plasmacytoma in BALB/c mice). The cells are resistant to 8-azaguanine (20 μg/mL) but sensitive to HAT. Due to a deficiency in 3-ketosteroid reductase activity, the cells have been reported to be cholesterol auxotrophs. This cell line was tested for being not infected by ectromelia virus (mousepox).

Age: N/A

Tissue: B Lymphocyte; Phlogocyte; Myeloma

Morphology: Lymphoblast

Growth Properties: Suspension

Doubling Time: ~16-26 hours

Biosafty: 1

Medium: DMEM(PM150210)+10% FBS+1% P/S(PB180120)

Subculturing: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2×10^5 viable cells/ml.

Ratio: 2×10^5 cells/mL

Renewal: Every 2 to 3 days

Cryopreservation: Freeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase

Culture Conditions: Atmosphere: Air, 95%; CO2, 5% Temperature: 37℃

Effects: N/A

Duration: N/A

Lead Time: 3-5 days