P3X63Ag8.653 Cell Line | CL-0410

P3X63Ag8.653 Cell Line | CL-0410 | Gentaur US, UK & Europe Disrtribition

Media: CM-0410

Organism: Mus musculus, Mouse

Application: N/A

Background: The cells are resistant to 8-azaguanine and are HAT sensitive.They can be used as fusion partners for producing hybridomas.The cells have been reported to be cholesterol auxotrophs due to a deficiency in 3-ketosteroid reductase activity.Tested and found negative for ectromelia virus(mousepox).

Age: N/A

Tissue: Myeloma

Morphology: Lymphoblast

Growth Properties: Suspension

Doubling Time: N/A

Biosafty: 1

Medium: RPMI-1640(PM150110)+10% FBS+1% P/S(PB180120)

Subculturing: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at the subcultivation ratio of 1:2-1:4.

Ratio: 1:2 to 1:4

Renewal: Every 2 to 3 days

Cryopreservation: Freeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase

Culture Conditions: Atmosphere: Air, 95%; CO2, 5% Temperature: 37℃

Effects: N/A

Duration: N/A

Lead Time: 3-5 days