P3/NSI/1-Ag4-1 [NS-1] cell line | CL-0409

P3/NSI/1-Ag4-1 [NS-1] cell line | CL-0409 | Gentaur US, UK & Europe Disrtribition

Media: CM-0409

Organism: Mus musculus, Mouse

Application: N/A

Background: This cell line is a subclone of P3X27. The cells synthesize Kappa light chains but not secret. The cells are resistant to 8-azaguanine (20 μg/mL) but sensitive to HAT. Due to a deficiency in 3-ketosteroid reductase activity, the cells have been reported to be cholesterol auxotrophs. This cell line was tested for being not infected by ectromelia virus (mousepox).

Age: N/A

Tissue: B Lymphocyte; Phlogocyte; Myeloma

Morphology: Lymphoblast

Growth Properties: Suspension

Doubling Time: ~24 hours

Biosafty: 1

Medium: DMEM(PM150210)+10% FBS+1% P/S(PB180120)

Subculturing: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at the subcultivation ratio of 1:2-1:4.

Ratio: 1:2 to 1:4

Renewal: Every 2 to 3 days

Cryopreservation: Freeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase

Culture Conditions: Atmosphere: Air, 95%; CO2, 5% Temperature: 37℃

Effects: N/A

Duration: N/A

Lead Time: 3-5 days