P19 [P-19] Cell Line | CL-0179

P19 [P-19] Cell Line | CL-0179 | Gentaur US, UK & Europe Disrtribition

Media: CM-0179

Organism: Mus musculus, Mouse

Application: Transfection host.

Background: The line can be cloned at high efficiency in medium containing 0.1mM 2-mercaptoethanol.The cells are pluripotent.The cell can be induced to differentiate into neural and glial like cells in the presence of 500 nM retinoic acid.In the presence of 0.5% to 1.0% dimethylsulfoxide(DMSO) the cells differentiate to form cardiac and skeletal muscle-like elements, but do not form neural or glial like cells.In the presence of both DMSO and retinoic acid, the cells differentiate as in the presence of retinoic acid alone.

Age: N/A

Tissue: Embryo

Morphology: Epithelial

Growth Properties: Adherent

Doubling Time: N/A

Biosafty: 1

Medium: MEMα(PM150421)+7.5% CS+2.5% FBS+1% P/S(PB180120)

Subculturing: Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.

Ratio: 1:2 to 1:4

Renewal: Every 2 to 3 days

Cryopreservation: Freeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase

Culture Conditions: Atmosphere: Air, 95%; CO2, 5% Temperature: 37℃

Effects: N/A

Duration: N/A

Lead Time: 3-5 days