OP9 Cell Line | CL-0176

OP9 Cell Line | CL-0176 | Gentaur US, UK & Europe Disrtribition

Media: CM-0176

Organism: Mus musculus, Mouse

Application: OP9 cells can be used to coculture mouse embryonic stem cells(ES cells) to induce the differentiation of embryonic stem(ES) cells into blood cells of erythroid, myeloid, and B cell lineages. Cocultivation with OP9 does not require exogenous growth factors

Background: The cells do not produce functional macrophage colony-stimulating factor(M-CSF) due to an osteopetrotic mutation in the gene encoding M-CSF. The presence of M-CSF had inhibitory effects on the differentiation of embryonic stem(ES) cells to blood cells other than macrophages.

Age: Embryo

Tissue: Bone marrow/stroma

Morphology: Fibroblast-like

Growth Properties: Adherent

Doubling Time: N/A

Biosafty: 1

Medium: MEMα(PM150421)+20% FBS+1% P/S(PB180120)

Subculturing: Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.

Ratio: 1:2 to 1:4

Renewal: Every 2 to 3 days

Cryopreservation: Freeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase

Culture Conditions: Atmosphere: Air, 95%; CO2, 5% Temperature: 37℃

Effects: N/A

Duration: N/A

Lead Time: 3-5 days