Description
Nucleophosmin Antibody / NPM1 | V7981-20UG | Gentaur US, UK & Europe Disrtribition
Family: Primary antibody
Formulation: 0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Format: Purified
Clone: rNPM1/1901
Host: Mouse
Clonality: Recombinant Mouse Monoclonal
Isotype: Mouse IgG1, kappa
Species Reactivity: Human
Application: IHC-P, WB, FACS, IF
Application Details: Immunohistochemistry (FFPE): 1-2ug/ml (not suitable for frozen tissue sample testing),Western blot: 1-2ug/ml,Flow cytometry: 1-2ug/million cells in 0.1ml
Application Note: Optimal dilution of the antibody should be determined by the researcher.
Purity: Protein G affinity chromatography
Description: Nucleophosmin (NPM) is predominantly localized in the nucleus of cells in most tissues. NPM is involved in ribosomal assembly and rRNA transport. It is an abundant protein that is highly phosphorylated by Cdc2 kinase during mitosis. This phosphoprotein moves between the nucleus and the cytoplasm. It is thought to be involved in several processes including regulation of the ARF/p53 pathway. A number of genes are fusion partners, in particular the anaplastic lymphoma kinase gene on chromosome 2. Mutations in exon 12 affecting the C-terminus of the protein are associated with an aberrant cytoplasmic location. Mutations in this gene are associated with acute myeloid leukemia. The antibody may be a useful aid for classification of acute myeloid leukemia.
Immunogen: A recombinant human partial protein (amion acids 185-287) was used as the immunogen for this recombinant Nucleophosmin antibody.
Storage: Store the recombinant Nucleophosmin antibody at 2-8oC (with azide) or aliquot and store at -20 °C or colder (without azide).
Localization: Nuclear, cytoplasmic
