Nucleophosmin Antibody / NPM1 | V7981-100UG

Nucleophosmin Antibody / NPM1 | V7981-100UG | Gentaur US, UK & Europe Disrtribition

Family: Primary antibody

Formulation: 0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Format: Purified

Clone: rNPM1/1901

Host: Mouse

Clonality: Recombinant Mouse Monoclonal

Isotype: Mouse IgG1, kappa

Species Reactivity: Human

Application: IHC-P, WB, FACS, IF

Application Details: Immunohistochemistry (FFPE): 1-2ug/ml (not suitable for frozen tissue sample testing),Western blot: 1-2ug/ml,Flow cytometry: 1-2ug/million cells in 0.1ml

Application Note: Optimal dilution of the antibody should be determined by the researcher.

Purity: Protein G affinity chromatography

Description: Nucleophosmin (NPM) is predominantly localized in the nucleus of cells in most tissues. NPM is involved in ribosomal assembly and rRNA transport. It is an abundant protein that is highly phosphorylated by Cdc2 kinase during mitosis. This phosphoprotein moves between the nucleus and the cytoplasm. It is thought to be involved in several processes including regulation of the ARF/p53 pathway. A number of genes are fusion partners, in particular the anaplastic lymphoma kinase gene on chromosome 2. Mutations in exon 12 affecting the C-terminus of the protein are associated with an aberrant cytoplasmic location. Mutations in this gene are associated with acute myeloid leukemia. The antibody may be a useful aid for classification of acute myeloid leukemia.

Immunogen: A recombinant human partial protein (amion acids 185-287) was used as the immunogen for this recombinant Nucleophosmin antibody.

Storage: Store the recombinant Nucleophosmin antibody at 2-8oC (with azide) or aliquot and store at -20 °C or colder (without azide).

Localization: Nuclear, cytoplasmic