Nucleophosmin Antibody / NPM1 | V3911-20UG

Nucleophosmin Antibody / NPM1 | V3911-20UG | Gentaur US, UK & Europe Disrtribition

Family: Primary antibody

Formulation: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

Format: Purified

Clone: NA24

Host Animal: Mouse

Clonality: Monoclonal (mouse origin)

Species Reactivity: Human

Application: IHC-P, IF, FACS

Buffer: N/A

Limitation: This Nucleophosmin antibody is available for research use only.

Purity: Protein G affinity chromatography

Description: Recognizes a 33kDa glycoprotein, identified as Nucleophosmin (NPM). It is predominantly localized in the nucleus of cells in most tissues. NPM is involved in ribosomal assembly and rRNA transport. It is an abundant protein that is highly phosphorylated by Cdc2 kinase during mitosis. This phosphoprotein moves between the nucleus and the cytoplasm. It is thought to be involved in several processes including regulation of the ARF/p53 pathway. A number of genes are fusion partners, in particular the anaplastic lymphoma kinase gene on chromosome 2. Mutations in exon 12 affecting the C-terminus of the protein are associated with an aberrant cytoplasmic location. Mutations in this gene are associated with acute myeloid leukemia. The antibody may be a useful aid for classification of acute myeloid leukemia.

Immunogen: A GST fusion protein containing the N-terminal portion of Nucleophosmin fused to a 14 amino acid portion of ALK protein was used as the immunogen for the Nucleophosmin antibody.

Storage: Store the Nucleophosmin antibody at 2-8oC (with azide) or aliquot and store at -20 °C or colder (without azide).