Nucleolin Antibody | V8771SAF-100UG

Nucleolin Antibody | V8771SAF-100UG | Gentaur US, UK & Europe Disrtribition

Family: Primary antibody

Formulation: 1 mg/ml in 1X PBS; BSA free, sodium azide free

Format: Purified

Clone: NCL/7014R

Host: Rabbit

Clonality: Recombinant Rabbit Monoclonal

Isotype: Rabbit IgG, kappa

Species Reactivity: Human. Does not react with Mouse, Rat or Cow. Other species reactivity not known.

Application: FACS, IF, WB, IHC-P

Application Details: Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT

Application Note: Optimal dilution of the Nucleolin antibody should be determined by the researcher.

Purity: Protein A affinity chromatography

Description: Recognizes a protein of ~76kDa, which is identified as Nucleolin (NCL). It is the major nucleolar phosphoprotein of growing eukaryotic cells. NCL is located mainly in dense fibrillar regions of the nucleolus. It is found associated with intranucleolar chromatin and pre-ribosomal particles. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly. This MAb can be used to stain the nucleoli in cell or tissue preparations and can be used as a marker of the nucleoli in subcellular fractions. It produces a speckled pattern in the nuclei of cells of normal and malignant cells.

Immunogen: Recombinant full length human protein was used as the immunogen for the Nucleolin antibody.

Storage: Store the Nucleolin antibody at 2-8oC (with azide) or aliquot and store at -20 °C or colder (without azide).

Localization: Nucleoli