NIH:OVCAR-3 [OVCAR3] Cell Line | CL-0178

NIH:OVCAR-3 [OVCAR3] Cell Line | CL-0178 | Gentaur US, UK & Europe Disrtribition

Media: CM-0178

Organism: Homo sapiens, Human

Application: transfection. OVCAR3 is an appropriate model system in which to study drug resistance in ovarian cancer and the presence of hormone receptors should be useful for the evaluation of hormonal therapy.

Background: Forms colonies in soft agar and has an abnormal karyotype.Resistant to clinically relevant concentrations of adriamycin, melphalan and cisplatin.Both cultured cells and xenografts exhibit androgen and estrogen receptors.Xenograft models have been used to show that treatment with 17 beta Estradiol can induce progesterone receptors in this human ovarian carcinoma.

Age: 60 years

Tissue: Ovary

Morphology: Epithelial

Growth Properties: Adherent

Doubling Time: N/A

Biosafty: 1

Medium: RPMI-1640(PM150110)+20% FBS+0.01mg/ml Insulin+1% P/S(PB180120)

Subculturing: Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.

Ratio: 1:2 to 1:4

Renewal: Every 2 to 3 days

Cryopreservation: Freeze medium: 60% Basal medium+30% FBS+10% DMSOStorage temperature: Liquid nitrogen vapor phase

Culture Conditions: Atmosphere: Air, 95%; CO2, 5%Temperature: 37℃

Effects: Yes, Forms colonies in soft agar; Yes, in nude mice inoculated subcutaneously with 10^7 cells. Tumors developed within 21 days at 100% frequency(5/5).

Duration: N/A

Lead Time: 3-5 days