Description
NFAT Luciferase-eGFP Reporter Lentivirus-78656-P | 78656-P | Gentaur US, UK & Europe Disrtribition
Category: Cell Signaling/Lentivirus
Application: Screen for activators or inhibitors of NFAT signaling pathways in transduced target cells. Generate NFAT Luciferase-eGFP Reporter stable cell line (puromycin resistant).
Background: NFAT (Nuclear factor of activated T-cells) is a family of transcription factors that has an important function in immune responses, for example by inducing the expression of various cytokines (such as IL-2-3-4 and TNF-alpha) in T cells. Members of the NFAT family have been found in many tissue types including heart, skeletal muscle and brain cells. Through their role in the immune system, NFATs are involved in inflammation and these transcription factors are considered promising therapeutic targets for a variety of diseases. NFAT is regulated by Ca2+ and the Ca2+/calmodulin-dependent serine phosphatase, calcineurin. NFAT proteins are phosphorylated and reside in the cytoplasm in resting cells; upon stimulation, they are dephosphorylated by calcineurin, translocate to the nucleus, and induce gene expression.
Description: The NFAT Luciferase-eGFP Reporter Lentiviruses are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to transduce almost all types of mammalian cells, including primary and non-dividing cells. The particles contain a firefly luciferase and eGFP cassette driven by the NFAT response element located upstream of the minimal TATA promoter (Figure 1) and a puromycin selection gene to generate stable clones. After transduction, activation of the NFAT signaling pathway in the target cells can be monitored by measuring the luciferase activity or eGFP expression.
Product Type: Lentivirus
Shippement Condition: -80°C
