Neurofilament Antibody (-Heavy) / NF-H / NEFH | V7413-20UG

Neurofilament Antibody (-Heavy) / NF-H / NEFH | V7413-20UG | Gentaur US, UK & Europe Disrtribition

Family: Primary antibody

Formulation: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

Format: Purified

Clone: NFHP-1R

Host: Rabbit

Clonality: Recombinant Rabbit Monoclonal

Isotype: Rabbit IgG, kappa

Species Reactivity: Human

Application: IHC-P, WB

Application Details: Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT,Prediluted IHC only format: incubate for 30 min at RT (1)

Application Note: The stated application concentrations are suggested starting points. Titration of the recombinant NF-H antibody may be required due to differences in protocols and secondary/substrate sensitivity.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Purity: Protein A affinity

Description: Neurofilaments make up the main structural elements of axons and dendrites and are found in neurons, peripheral nerves, and sympathetic ganglion cells. Neurofilaments consist of three major subunits with molecular weights of 68kDa (NF-L), 160kDa (NF-M) and 200kDa (NF-H).

Immunogen: Recombinant human protein (heavy subunit) was used as the immunogen for the recombinant NF-H antibody.

Storage: Store the recombinant NF-H antibody at 2-8oC (with azide) or aliquot and store at -20 °C or colder (without azide).

Localization: Cytoplasmic, membranous