Description
NeuN Antibody / Fox3 | V9368-20UG | Gentaur US, UK & Europe Disrtribition
Family: Primary antibody
Formulation: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Format: Purified
Clone: NeuN/288R
Host: Rabbit
Clonality: Recombinant Rabbit Monoclonal
Isotype: Rabbit IgG, kappa
Species Reactivity: Human
Application: IHC-P, WB
Application Details: Immunohistochemistry (FFPE): 1-2ug/ml,Western blot:1-2ug/ml
Application Note: Optimal dilution of the recombinant NeuN antibody should be determined by the researcher.
Purity: Protein A/G affinity
Description: RNA binding protein fox-1 homolog 3, also called RBFOX3, Neuronal Nuclei Antigen and NeuN, is present in most CNS and PNS neuronal cell types of all vertebrates tested. NeuN protein distributions are apparently restricted to neuronal nuclei and some proximal neuronal processes in both fetal and adult brain although, some neurons fail to be recognized by NeuN at all ages: INL retinal cells, Cajal-Retzius cells, Purkinje cells, inferior olivary and dentate nucleus neurons, and sympathetic ganglion cells are examples. Immunohistochemically detectable NeuN protein first appears at developmental timepoints that correspond with the withdrawal of the neuron from the cell cycle and/or with the initiation of terminal differentiation of the neuro. Immunoreactivity appears around E9.5 in the mouse neural tube and is extensive throughout the developing nervous system by E12.5. Strong nuclear staining suggests a nuclear regulatory protein function; however, no evidence currently exists as to whether the NeuN protein antigen has a function in the distal cytoplasm or whether it is merely synthesized there before being transported back into the nucleus. No difference between protein isolated from purified nuclei and whole brain extract on immunoblots has been found.
Immunogen: Recombinant full-length human RNA binding protein fox-1 homolog 3 protein was used as the immunogen for the recombinant NeuN antibody.
Storage: Aliquot the recombinant NeuN antibody and store frozen at -20 °C or colder. Avoid repeated freeze-thaw cycles.
Localization: Nucleus, Cytoplasm
