Description
NEDD8 E1, His-tag | 81087 | Gentaur US, UK & Europe Disrtribition
Category: Ubiquitination/Activating Enzyme (E1)
Application: Useful for NEDD8 conjugation reactions and NEDDylation pathway studies
Background: The NEDD8 activating E1 enzyme is a heterodimer composed of APPBP1 and UBA3 subunits. The APPBP1/UBA3 enzyme has homology to the N- and C-terminal halves of the ubiquitin E1 enzyme, respectively. The UBA3 subunit contains the catalytic center and activates NEDD8 in an ATP-dependent reaction by forming a high-energy thiolester intermediate. The activated NEDD8 is subsequently transferred to the UbcH12 E2 enzyme, and is then conjugated to specific substrates in the presence of the appropriate E3 ligases.
Description: Human NEDD8 E1 recombinant protein complex, with His-tagged APPBP1 and untagged Uba3, co-expressed in E. coli.
Product Type: Protein
Shippement Condition: -80°C (dry ice)
