Description
NCI-H520 cell line | CL-0402 | Gentaur US, UK & Europe Disrtribition
Media: CM-0402
Organism: Homo sapiens, Human
Application: N/A
Background: The cell line was established in 1982 from a sample of a lung mass taken by A.F. Gazdar from a patient with squamous cell carcimoma of the lung. A greatly reduced level of p53 mRNA is expressed by this cell line compared to normal lung tissue. The cells exhibit no gross structural DNA abnormalities. The cells stain positive for keratin and vimentin but negative for neurofilament triplet protein. The cells can form colonies in soft agar with/without serum.
Age: N/A
Tissue: Lung
Morphology: Epithelial
Growth Properties: Adherent
Doubling Time: ~32-60 hours
Biosafty: 1
Medium: RPMI-1640(PM150110)+10% FBS+1% P/S(PB180120)
Subculturing: Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
Ratio: 1:3 to 1:4
Renewal: 2 to 3 times per week
Cryopreservation: Freeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase
Culture Conditions: Atmosphere: Air, 95%; CO2, 5% Temperature: 37℃
Effects: N/A
Duration: N/A
Lead Time: 3-5 days
