NCI-H508[H508] Cell Line | CL-0702

NCI-H508[H508] Cell Line | CL-0702 | Gentaur US, UK & Europe Disrtribition

Media: CM-0702

Organism: Homo sapiens, Human

Application: N/A

Background: This line was derived from a metastasis to the abdominal wall obtained from a patient after treatment with 5-fluorouracil.

Age: 55 years

Tissue: Large intestine; Cecum

Morphology: Epithelial

Growth Properties: Mixed: floating aggregates of round cells with some attached cells

Doubling Time: 32 hours

Biosafty: 1

Medium: RPMI-1640(PM150110)+10%FBS+1%P/S(PB180120)

Subculturing: Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. Remove culture medium. Keep floating cells. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C.to facilitate dispersal. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension and the harvested cells to new culture vessels. Incubate cultures at 37°C.

Ratio: 1:2 to 1:4

Renewal: 3 times per week

Cryopreservation: Freeze Medium: 55% Basal Medium+40% FBS+5% DMSO Storage temperature: Liquid nitrogen vapor phase

Culture Conditions: Atmosphere: Air, 95%; CO2, 5% Temperature: 37℃

Effects: N/A

Duration: N/A

Lead Time: 3-5 days