NCI-H23 cell line | CL-0397

NCI-H23 cell line | CL-0397 | Gentaur US, UK & Europe Disrtribition

Media: CM-0397

Organism: Homo sapiens, Human

Application: N/A

Background: This line was established from lung cancer tissue obtained from a 59-year-old black male lung adenocarcinoma patient prior to therapy. The cells carry the mutation of K-ras 12 and a mutation in the codon 246 (ATC →ATG, isoleucine -> methionine) of the p53 gene. The cells express C-myc, L-myc, v-src, v-abl, v-erb B, c-raf 1, Ha-ras, Ki-ras and N-ras RNAs. The cell line has a heterogeneous mRNA expression for PDGF A and B chain, transforming growth factor alpha and beta and the epidermal growth factor receptor (EGFR). NCI-H23 exhibits a 20-fold higher level of c-myc DNA amplification without detectable c-myc RNA amplification. The cells stain positive for keratins 5+8 and 18 and vimentin but negative for neurofilament. The cells are L-dopa decarboxylase-negative. The cells can form colonies in soft agarose with an efficiency of 9.7%.

Age: male, 51 years

Tissue: Lung

Morphology: Epithelial

Growth Properties: Adherent

Doubling Time: ~38 hours

Biosafty: 1

Medium: RPMI-1640(PM150110)+10% FBS+1% P/S(PB180120)

Subculturing: Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.

Ratio: 1:3 to 1:4

Renewal: Every 2 to 3 days

Cryopreservation: Freeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase

Culture Conditions: Atmosphere: Air, 95%; CO2, 5% Temperature: 37℃

Effects: N/A

Duration: N/A

Lead Time: 3-5 days