NCI-H146 [H146] Cell Line | CL-0693

NCI-H146 [H146] Cell Line | CL-0693 | Gentaur US, UK & Europe Disrtribition

Media: CM-0693

Organism: Homo sapiens, Human

Application: N/A

Background: This is a near triploid human cell line. The modal chromosome number is 68, but cells with 66, 70 and 71 chromosomes also occurred frequently. At least 15 markers were common to all cells including single copies of der(1)t(1;13)(p32;q12) and del(3)(p21/22), and paired der(7)t(7;?)(p22;?), t(13q16q) and t(16p21q). Ten others were seen in single copies or paired. No HSR or DM were found. Structurally normal N5, N13 and N14 were absent. Four or more copies of N18 and N20 were present in each cell, and N5, N7 and N11 were mostly single copies per cell. The X chromosomes were paired, and no Y chromosome was detected in QM stained preparations

Age: 59 years

Tissue: Lung

Morphology: Epithelial

Growth Properties: Suspension

Doubling Time: N/A

Biosafty: 1

Medium: RPMI-1640(PM150110)+10%FBS+1%P/S(PB180120)

Subculturing: The line should be subcultured by dilution with fresh medium. Alternatively, the clusters may be collected by centrifugation and resuspended in fresh medium.

Ratio: 1:2 to 1:6

Renewal: 2 to 3 times per week

Cryopreservation: Freeze Medium: 55% Basal Medium+40% FBS+5% DMSO Storage temperature: Liquid nitrogen vapor phase

Culture Conditions: Atmosphere: Air, 95%; CO2, 5% Temperature: 37℃

Effects: N/A

Duration: N/A

Lead Time: 3-5 days