Description
MVP Antibody / Major Vault Protein | V7393SAF-100UG | Gentaur US, UK & Europe Disrtribition
Family: Primary antibody
Formulation: 1 mg/ml in 1X PBS; BSA free, sodium azide free
Format: Purified
Clone: VP2897R
Host: Rabbit
Clonality: Recombinant Rabbit Monoclonal
Isotype: Rabbit IgG, kappa
Species Reactivity: Human
Application: IHC-P
Application Details: Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
Application Note: Optimal dilution of the recombinant MVP antibody should be determined by the researcher.
1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Purity: Protein A affinity chromatography
Description: Recognizes a protein of 104kDa-110kDa, characterized as major vault protein (MVP). Vaults are large ribonucleoprotein particles (RNPs) present in all eukaryotic cells. They have a complex morphology, including several small molecules of RNA, but a single protein species. The MVP accounts for >70% of their mass. Their shape is reminiscent of the nucleopore central plug. Treatment of cells with estradiol increases the amount of MVP in nuclear extract. The hormone-dependent interaction of vaults with ER is prevented in vitro by sodium molybdate. Antibodies to estrogen, progesterone and glucocorticoid receptors are able to co-immunoprecipitate the MVP. MVP is overexpressed in many neoplastic tissues and cell lines. Expression of MVP predicts a poor response to chemotherapy.
Immunogen: Recombinant human protein was used as the immunogen for the recombinant MVP antibody.
Storage: Store the recombinant MVP antibody at 2-8oC (with azide) or aliquot and store at -20 °C or colder (without azide).
Localization: Cytoplasmic, nuclear
