MUC1 Antibody / Mucin-1 | V7311-20UG

MUC1 Antibody / Mucin-1 | V7311-20UG | Gentaur US, UK & Europe Disrtribition

Family: Primary antibody

Formulation: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

Format: Purified

Clone: MUC1/2818R

Host: Rabbit

Clonality: Recombinant Rabbit Monoclonal

Isotype: Rabbit IgG, kappa

Species Reactivity: Human

Application: ELISA, IHC-P, WB

Application Details: ELISA: 2-5ug/ml (order BSA/sodium azide-free format),Immunohistochemistry (FFPE): 0.25-0.5ug/ml for 30 min at RT,Western blot: 1-2ug/ml

Application Note: Titration of the recombinant Mucin-1 may be required due to differences in protocols and secondary/substrate sensitivity.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Purity: Protein A affinity chromatography

Description: MUC1 is a large cell surface mucin glycoprotein expressed by most glandular and ductal epithelial cells and some hematopoietic cell lineages. It is expressed on most secretory epithelium, including mammary gland and some hematopoietic cells. It is expressed abundantly in lactating mammary glands and over expressed abundantly in >90% breast carcinomas and metastases. Transgenic MUC1 has been shown to associate with all four c-erbB receptors and localize with c-erbB1 (EGFR) in lactating glands. The MUC1 gene contains seven exons and produces several different alternatively spliced variants. The major expressed form of MUC1 uses all seven exons and is a type 1 transmembrane protein with a large extracellular tandem repeat domain. The tandem repeat domain is highly O glycosylated and alterations in glycosylation have been shown in epithelial cancer cells. Antibody to MUC1 is useful as a pan-epithelial marker for detecting early metastatic loci of carcinoma in bone marrow or liver.

Immunogen: Human milk fat globule membranes were used as the immunogen for this recombinant Mucin-1 antibody.

Storage: Store the recombinant Mucin-1 antibody at 2-8oC (with azide) or aliquot and store at -20 °C or colder (without azide).

Localization: Cytoplasmic and cell surface