Description
MSTO-211H cell line | CL-0163 | Gentaur US, UK & Europe Disrtribition
Media: CM-0163
Organism: Homo sapiens, Human
Application: N/A
Background: This cell line was established in 1985 from the the pleural effusion of metastatic site on biphasic mesothelioma. The 62-year-old male Caucasian patient had not received prior radiation or chemotherapy. The cells have EGF binding sites with high binding affinity. The cells express alpha- and beta-subunits of human chorionic gonadotropin as well as Neuron specific enolase. L-DOPA decarboxylase, bombesin and neurotensin were not detected in the cells. C-myc protooncogene is overexpressed by the cells with no gene rearrangement/amplification observed. The cells are positive for the expression of v-src, v-abl, v-erb B, c-raf 1, Ha-ras, Ki-ras, and N-ras. No detectable expression of N-myc, L-myc, c-myb, c-fos, v-fes, v-fms and v-sis. A saturation cell density of 400,000 cell/cm2 can be reached with cell surface slough off.
Age: male, 62 years
Tissue: Lung; Derived From Metastatic Site: Pleural Effusion
Morphology: Fibroblast-Like
Growth Properties: Adherent
Doubling Time: ~30-40 hours
Biosafty: 1
Medium: RPMI-1640(PM150110)+10% FBS+1% P/S(PB180120)
Subculturing: Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
Ratio: 1:3 to 1:4
Renewal: Every 2 to 3 days
Cryopreservation: Freeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase
Culture Conditions: Atmosphere: Air, 95%; CO2, 5% Temperature: 37℃
Effects: N/A
Duration: N/A
Lead Time: 3-5 days
