MRC-5 Cell Line | CL-0161

MRC-5 Cell Line | CL-0161 | Gentaur US, UK & Europe Disrtribition

Media: CM-0161

Organism: Homo sapiens, Human

Application: N/A

Background: The MRC-5 cell line was derived from normal lung tissue of a 14-week-old male fetus in 1966. The cells are capable of 42 to 46 population doublings before the onset of senescence. This is a normal diploid human cell line with 46, XY karyotype. The modal chromosome number was 46, occurring in 70% of cells.

Age: Male, 14FW

Tissue: Lung

Morphology: Fibroblast

Growth Properties: Adherent

Doubling Time: 36-96 hours

Biosafty: 1

Medium: MEM(PM150410)+10% FBS+1% P/S(PB180120)

Subculturing: Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.

Ratio: 1:2 to 1:4

Renewal: 1 to 2 times per week

Cryopreservation: Freeze Medium: 55% Basal Medium+40% FBS+5% DMSOStorage Temperature: Liquid Nitrogen Vapor Phase

Culture Conditions: Atmosphere: Air, 95%; CO2, 5%;Temperature: 37℃

Effects: N/A

Duration: N/A

Lead Time: 3-5 days