Description
MRC-5 Cell Line | CL-0161 | Gentaur US, UK & Europe Disrtribition
Media: CM-0161
Organism: Homo sapiens, Human
Application: N/A
Background: The MRC-5 cell line was derived from normal lung tissue of a 14-week-old male fetus in 1966. The cells are capable of 42 to 46 population doublings before the onset of senescence. This is a normal diploid human cell line with 46, XY karyotype. The modal chromosome number was 46, occurring in 70% of cells.
Age: Male, 14FW
Tissue: Lung
Morphology: Fibroblast
Growth Properties: Adherent
Doubling Time: 36-96 hours
Biosafty: 1
Medium: MEM(PM150410)+10% FBS+1% P/S(PB180120)
Subculturing: Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
Ratio: 1:2 to 1:4
Renewal: 1 to 2 times per week
Cryopreservation: Freeze Medium: 55% Basal Medium+40% FBS+5% DMSOStorage Temperature: Liquid Nitrogen Vapor Phase
Culture Conditions: Atmosphere: Air, 95%; CO2, 5%;Temperature: 37℃
Effects: N/A
Duration: N/A
Lead Time: 3-5 days
