Description
MPG Antibody | R30923 | Gentaur US, UK & Europe Disrtribition
Family: Primary antibody
Formulation: 0.5mg/ml if reconstituted with 0.2ml sterile DI water
Format: Antigen affinity purified
Clone: N/A
Host Animal: Rabbit
Clonality: Polyclonal (rabbit origin)
Species Reactivity: Human, Mouse, Rat
Application: WB
Buffer: N/A
Limitation: This MPG antibody is available for research use only.
Purity: Antigen affinity
Description: N-Methylpurine-DNA glycosylase is encoded by the MPG gene, mapped to human chromosome 16 by analysis of a panel of DNAs from mouse/human and hamster/human hybrid cell lines. The gene was expressed in all cell lines and tissues examined, but was found at particularly high levels in a colon adenocarcinoma cell line (HT29). The completely characterized human gene was found to span 7 to 8 kb of genomic DNA and to be localized 75 kb upstream of the embryonic zeta-globin gene. To assess the contribution of MPG to the repair of several mutagenic lesions in vivo, Hang et al.(1997) biochemically analyzed cell-free extracts of tissues from mice with a targeted deletion of the gene. Following treatment with DNA-methylating agents, increased persistence of 7-methylguanine (meG) was found in liver sections of MPG knockout mice in comparison with wildtype mice, demonstrating an in vivo phenotype for the MPG-null animals.
Immunogen: An amino acid sequence from the C-terminus of human MPG (NKSFDQRDLAQDEAVW) was used as the immunogen for this MPG antibody.
Storage: After reconstitution, the MPG antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20 °C. Avoid repeated freezing and thawing.