Mouse PD-L1/TCR Activator Mammalian Expression Kit | 79778

Mouse PD-L1/TCR Activator Mammalian Expression Kit | 79778 | Gentaur US, UK & Europe Disrtribition

Category: Immunotherapy/Cell Based Assay Kit

Application: • Screen for activators or inhibitors of mouse PD-1 signaling in a cellular context
• Characterize the biological activity of mouse PD-1 and its interactions with ligands

Background: The binding of Programmed Cell Death Protein 1 (PD-1), a receptor expressed on activated T-cells, to its ligands, PD-L1 and PD-L2, negatively regulates immune responses. The PD-1 ligands are found on most cancers, and PD-1:PD-L1/2 interaction inhibits T cell activity and allows cancer cells to escape immune surveillance. The PD-1:PD-L1/2 pathway is also involved in regulating autoimmune responses, making these proteins promising therapeutic targets for a number of cancers, as well as multiple sclerosis, arthritis, lupus, and type I diabetes.

Description: The recombinant expression vectors are designed to express human engineered T cell receptor (TCR) activator and mouse PD-L1 (GenBank Accession #NM_021893) in mammalian cells. The transfected cells can be used in conjunction with mouse PD-1/NFAT Reporter/Jurkat T cells (BPS Bioscience #79762) to study the interactions of PD-1 with PD-L1 ligand in a cellular context and to screen for modulators of this signaling pathway. Functional Validation and Assay Performance: In this assay, mouse PD-1/NFAT Reporter/Jurkat T cells are used as effector cells; HEK293 cells transiently transfected with the PD-L1 vector over-express mouse PD-L1 and an engineered T cell receptor (TCR) activator and are used as target cells. When these two cells are co-cultivated, TCR complexes on effector cells are activated by TCR activator on target cells, resulting in expression of the NFAT luciferase reporter. However, PD1 and PD-L1 ligation prevents TCR activation and suppresses the NFAT-responsive luciferase activity. This inhibition can be specifically reversed by anti-PD1 or anti-PD-L1 antibodies. Mouse PD1/PD-L1 neutralizing antibodies block PD1:PD-L1 interaction and promote T cell activation, resulting in reactivation of the NFAT responsive luciferase reporter.

Product Type: Reporter Assay Kit

Shippement Condition: -20°C