Description
Mouse LRRC32&TGF-beta 1 Heterotrimer protein, His Tag&Tag Free (MALS verified) | GA1-M52W2-1mg| Gentaur Distribution US, UK & Europe
GARP (LRRC32) is a transmembrane protein that binds latent-TGF-β1 and tethers it on the Treg surface. and has been proved to promote the activation and secretion of transforming growth factor β (TGF-β). The expression of GARP is highly on the surface activated Tregs and increases the suppressive function of Tregs. Additionally, GARP can bind to latent transforming growth factor β (TGF-β), thus promoting secretion and activation of TGF-β. TGF-β plays a critical rule for homeostasis and function of Tregs. Notably, it has been also observed that fibroblasts and endothelial cell lines that express GARP/latent TGF-β1 complexes do not activate TGF-β1. However, it cannot be excluded that specific stimuli are required to trigger TGF-β1 activation from complexes on the surface of these cell types.
Source: Mouse LRRC32&TGFB1 Heterotrimer protein, His Tag&Tag Free (GA1-M52W2) is expressed from human 293 cells (HEK293). It contains AA Ile 18 - Asn 628 (LRRC32) & Leu 30 - Ser 390 (TGF-beta 1) (Accession # G3XA59-1 (LRRC32) & P04202-1 (TGF-beta 1) ).
Format: Powder.
Tag: C-10×His | Native.
Expression System: HEK293.
Expression Region: Ile 18 - Asn 628 (LRRC32) & Leu 30 - Ser 390 (TGF-beta 1).
Conjugate: Unconjugated.
Molecular Weight: 68.7 kDa | 28.5 kDa & 12.8 kDa.
Characteristics: Mouse LRRC32&TGFB1 Heterotrimer Protein, His Tag&Tag Free (the molar ratio of LRRC32 & TGF-beta 1 equals 1:2) is produced by co-expression of LRRC32 and TGF-beta 1, which has a calculated MW of 68.7 kDa (LRRC32), 28.5 (LAP) and 12.8 kDa (mature TGF-beta 1) respectively. LRRC32 is fused with a polyhistidine tag at the C-terminus and TGF-beta 1 contains no tag. The reducing (R) Heterotrimer protein migrates as 80 kDa (LRRC32), 40 kDa (LAP) and 14 kDa (mature TGF-beta 1) due to glycosylation respectively.
Purity: 95%.
Buffer: PBS, pH7.4.
Storage Conditions: -20℃.
Shipping Conditions: RT
