Mouse Cardiac Fibroblasts | CP-M074

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SKU:
575-CP-M074
€1,040.00
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Description

Mouse Cardiac Fibroblasts | CP-M074 | Gentaur US, UK & Europe Disrtribition

Abbreviation: M-CF

Organism: Mouse

Tissue Type: Cardiovascular system

Tissue: Cardiac tissue

Cell Type: Fibroblast

Growth Proprieties: Adherent

Medium: CM-M074

Ratio: 1:2-1:3

Renwal: Every 2-3 days

Background: Mouse cardiac fibroblasts are isolated from cardiac tissue. The heart is composed of cardiomyocytes and non-cardiomyocytes. Non-cardiomyocytes account for 70% of the total number of cells, and more than 90% of the non-cardiomyocytes are composed of cardiac fibroblasts. Fibroblasts are the main cellular components of loose connective tissue, which are derived from the mesenchyme in the embryonic period. Fibroblasts are large, with clear outlines, mostly protruding spindle-shaped or star-shaped flat structures, with regular oval nuclei and large and obvious nucleoli. Fibroblasts have vigorous functions and activities. The cytoplasm is weakly basophilic and has obvious protein synthesis and secretion activities. Under certain conditions, it can achieve mutual transformation with fibroblasts. Fibroblasts are very important for the repair of different degrees of cell degeneration, necrosis and tissue defects effect. The newly isolated lung fibroblasts are round and have good refractive index. They are suspended in the culture medium. After 30 minutes, the cells adhere to the flask, and some of them begin to protrude pseudopods, which appear as small protrusions. After 6 hours, the cells are almost completely attached. It stretches into a fusiform with clear nuclei, relatively uniform distribution, scattered growth, and does not aggregate into clusters. The cells grow rapidly and reach confluence within 5-7 days. The cells are tightly arranged, and some overlap and grow, flat and large. The cytoplasm is transparent, the nucleus is large, elliptical, and light in color. The cells reach confluence and connect to each other into a network. The cells are distributed in a protruding spindle or star-shaped flat. In recent years, people have gradually learned that non-cardiomyocytes not only have structural support and protection for cardiomyocytes, but also have autocrine and paracrine functions, which affect the structure and physiological functions of cardiomyocytes. The main function of myocardial fibroblasts is to support the structure of myocardial cells and is responsible for the synthesis outside the cell matrix. When the myocardium is injured, it can produce paracrine growth factors. Cardiac fibroblasts are the most common cells in the connective tissue of the heart, which can synthesize and secrete collagen fibers, elastic fibers, reticular fibers and organic matrix. In addition, under the stimulation of trauma and other factors, some fibroblasts can be transformed into naive fibroblasts, their functional activities are also restored, and they participate in the repair of tissues after injury. Therefore, the physiology of cardiac fibroblasts has become a focus of modern cardiovascular research. The mouse myocardial fibroblasts produced by our company are prepared by trypsin-collagenase mixed digestion method combined with differential adhesion method, the total number of cells is about 5×10^5 cells/vial. The cells are identified by Vimentin immunofluorescence, and the purity is more than 90% without HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi, etc.

Delivery: 5 weeks

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