Mouse BACE1(Beta-Site APP Cleaving Enzyme 1) ELISA Kit | E-EL-M0182

Mouse BACE1(Beta-Site APP Cleaving Enzyme 1) ELISA Kit | E-EL-M0182 | Gentaur US, UK & Europe Disrtribition

Target Species: Mouse

Type: Sandwich

Assay Time: 3.5h

Detection Type: Colormetric

Sensitivitys: 0.1ng/mL

Detection Range: 0.16~10ng/mL

Target Name: BACE1

Target Synonyms: ASP2, HSPC104, Beta Secretase 1, Memapsin 2, membrane-associated aspartic protease 2, aspartyl protease 2

Tested Sample: Serum, plasma and other biological fluids

Test Principale: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse BACE1. Samples (or Standards) are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse BACE1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse BACE1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 ± 2 nm. The OD value is proportional to the concentration of Mouse BACE1. You can calculate the concentration of Mouse BACE1 in the samples by comparing the OD of the samples to the standard curve.

Specificity: This kit recognizes Mouse BACE1 in samples. No significant cross-reactivity or interference between Mouse BACE1 and analogues was observed.

Application: This ELISA kit applies to the in vitro quantitative determination of Mouse BACE1 concentrations in Serum, plasma and other biological fluids.

Research Area: Cancer, Cell Biology, Neuroscience

Storage : An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the items separately according to the storage table in the manual once the kit is received.