Description
MOLT-4 Cell Line | CL-0160 | Gentaur US, UK & Europe Disrtribition
Media: CM-0160
Organism: Homo sapiens, Human
Application: Transfection.
Background: The patient had received prior multidrug chemotherapy.There is a G-】 A mutation at codon 248 of the p53 gene.P53 is not expressed.The cells do not produce immunoglobulin or Epstein-Barr virus.The line was originally contaminated with mycoplasma but has been cured with antibiotics.
Age: 19 years
Tissue: Peripheral blood
Morphology: Lymphoblast
Growth Properties: Suspension
Doubling Time: N/A
Biosafty: 1
Medium: RPMI-1640(PM150110)+10% FBS+1% P/S(PB180120)
Subculturing: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2×10^5 viable cells/ml. Maintain cell density between 1×10^5 and 1×10^6 viable cells/ml.
Ratio: 1:2 to 1:4
Renewal: Every 2 to 3 days
Cryopreservation: Freeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase
Culture Conditions: Atmosphere: Air, 95%; CO2, 5% Temperature: 37℃
Effects: Yes, in untreated nude mice, anti lymphocyte serum treated mice and X-irradiated mice.
Duration: N/A
Lead Time: 3-5 days
