MOLT-4 Cell Line | CL-0160

MOLT-4 Cell Line | CL-0160 | Gentaur US, UK & Europe Disrtribition

Media: CM-0160

Organism: Homo sapiens, Human

Application: Transfection.

Background: The patient had received prior multidrug chemotherapy.There is a G-】 A mutation at codon 248 of the p53 gene.P53 is not expressed.The cells do not produce immunoglobulin or Epstein-Barr virus.The line was originally contaminated with mycoplasma but has been cured with antibiotics.

Age: 19 years

Tissue: Peripheral blood

Morphology: Lymphoblast

Growth Properties: Suspension

Doubling Time: N/A

Biosafty: 1

Medium: RPMI-1640(PM150110)+10% FBS+1% P/S(PB180120)

Subculturing: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2×10^5 viable cells/ml. Maintain cell density between 1×10^5 and 1×10^6 viable cells/ml.

Ratio: 1:2 to 1:4

Renewal: Every 2 to 3 days

Cryopreservation: Freeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase

Culture Conditions: Atmosphere: Air, 95%; CO2, 5% Temperature: 37℃

Effects: Yes, in untreated nude mice, anti lymphocyte serum treated mice and X-irradiated mice.

Duration: N/A

Lead Time: 3-5 days