Mitochondrial Marker Antibody | V7473-20UG

Mitochondrial Marker Antibody | V7473-20UG | Gentaur US, UK & Europe Disrtribition

Family: Primary antibody

Formulation: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

Format: Purified

Clone: MTC02/2860R

Host: Rabbit

Clonality: Recombinant Rabbit Monoclonal

Isotype: Rabbit IgG, kappa

Species Reactivity: Human

Application: IHC-P

Application Details: Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT

Application Note: The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the recombinant Mitochondrial Marker antibody to be titered up or down for optimal performance.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Purity: Protein A affinity chromatography

Description: This MAb recognizes a 60kDa antigen associated with the mitochondria in human cells. It can be used to stain mitochondria in cell or tissue preparations and can be used as a mitochondrial marker in subcellular fractions. It produces a spaghetti-like pattern in normal and malignant cells. This antibody is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells, including neurons and embryonic stem cells. Immunostaining pattern with anti-mitochondrial MAb has been reported as a useful discriminatory adjunct in the complex differential diagnosis of granular renal cell tumors.

Immunogen: A semi-purified mitochondria preparation was used as the immunogen for this recombinant Mitochondrial Marker antibody.

Storage: Store the recombinant Mitochondrial Marker antibody at 2-8oC (with azide) or aliquot and store at -20 °C or colder (without azide).

Localization: Mitochondria in cytoplasm