Description
MIA PaCa-2 cell line | CL-0627 | Gentaur US, UK & Europe Disrtribition
Media: CM-0627
Organism: Homo sapiens, human
Application: N/A
Background: The MIA PaCa-2 cell line was established by A. Yunis, et al. in 1975 from tumor tissue of the pancreas obtained from a 65-year-old Caucasian male.
Age: Male,65 years
Tissue: Pancreas
Morphology: Attached epithelial with floating rounded cells
Growth Properties: Adherent, single cells and loosely attached clusters
Doubling Time: ~40
Biosafty: 1
Medium: DMEM(PM150210)+10%FBS+2.5%HS+1%P/S(PB180120)
Subculturing: Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
Ratio: 1:2 to 1:5
Renewal: Every 2 to 3 days
Cryopreservation: Freeze Medium: 55% Basal Medium+40% FBS+5% DMSOStorage Temperature: Liquid Nitrogen Vapor Phase
Culture Conditions: Atmosphere: Air, 95%; CO2, 5%;Temperature: 37℃
Effects: N/A
Duration: N/A
Lead Time: 3-5 days
