MGMT Polyclonal Antibody | E-AB-70262

MGMT Polyclonal Antibody | E-AB-70262 | Gentaur US, UK & Europe Disrtribition

Reactivity: Mouse, Rat

Host: Rabbit

Isotype: IgG

Application: IHC

Research Area: Cancer, Epigenetics and Nuclear Signaling

Background: MGMT (O6-methylguanine-DNA methyltransferase) is transcriptionally activated in response to DNA damage and functions to repair mutagenic and cytotoxic O6-alkylguanine lesions caused by carcinogens or cytostatic drugs. MGMT induction by ionising radiation does not occur in p53-deficient mice, suggesting that MGMT induction may require p53. Similarly, MGMT mRNA and protein were shown to be inducible by ionising radiation, only in cell lines that express functional p53, and not in cell lines that do not express wild type p53. In contrast, high MGMT activity was associated with the presence of mutant p53, in a study of oral cancer cell lines. Similarly, MGMT activity was significantly lower in ovarian tumors with wildtype p53 than in tumors with mutant p53, supporting the view that wildtype p53 down-regulates the basal MGMT promoter.

Lead Time: 7~10 days

Concentration: 260 μg/mL

Storage: Store at -20°C. Avoid freeze / thaw cycles.

Immunogen: Recombinant protein corresponding to Mouse MGMT

Buffer: PBS with 0.02% sodium azide, 1% BSA and 50% glycerol, pH7.4

Purification: Affinity purification

Dilution: IHC 1:100-1:500

Conjugation: Unconjugated

Calculated Molecular Weight: N/A

Observed Molecular Weight: N/A

Clonality: Polyclonal