Description
MGMT Antibody | V4036SAF-100UG | Gentaur US, UK & Europe Disrtribition
Family: Primary antibody
Formulation: 1 mg/ml in 1X PBS; BSA free, sodium azide free
Format: Purified
Clone: MGMT/4791
Host Animal: Mouse
Clonality: Monoclonal (mouse origin)
Species Reactivity: Human
Application: IHC-P
Buffer: N/A
Limitation: This MGMT antibody is available for research use only.
Purity: Protein A/G affinity
Description: Cancer chemotherapeutic alkylating agents (e.g. BCNU,) act by inducing formation of lethal cross links at the O6 alkylguanine position in DNA. MGMT transfers alkyl adducts from the O6 position of guanine in DNA (prior to cross link formation) to a cysteine residue in its own sequence, thereby restoring DNA to its intact state. This transfer inactivates the MGMT enzyme and is irreversible; hence the level of MGMT in a cell is directly proportional to the level of DNA damage it can tolerate. In normal tissues, MGMT acts as a suppressor of mutation and carcinogenesis. Tumors with high levels of MGMT are likely to be drug resistant
Immunogen: Purified His-tagged MGMT protein was used as the immunogen for the MGMT antibody.
Storage: Aliquot the MGMT antibody and store frozen at -20 °C or colder. Avoid repeated freeze-thaw cycles.
