Description
MERS Spike protein trimer (R748A, R751A, V1060P, L1061P), His Tag (MALS verified) | SPN-M52H5-50ug| Gentaur Distribution US, UK & Europe
It's been reported that SARS-CoV-2 can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.
Source: MERS Spike protein trimer (R748A, R751A, V1060P, L1061P), His Tag (SPN-M52H5) is expressed from human 293 cells (HEK293). It contains AA Tyr 18 - Trp 1295 (Accession # K0BRG7 (R748A, R751A, V1060P, L1061P) ). The recombinant protein has a T4 fibritin trimerization motif. Proline substitutions (V1060P, L1061P) and alanine substitutions (R748A, R751A) are introduced to stabilize the trimeric prefusion state of MERS-CoV Spike protein and abolish the furin cleavage site, respectively.
Format: Powder.
Tag: C-10×His.
Expression System: HEK293.
Expression Region: Tyr 18 - Trp 1295.
Conjugate: Unconjugated.
Molecular Weight: 145.9 kDa.
Characteristics: This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 145.9 kDa. The protein migrates as 150 kDa and 175-200 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Purity: 90%.
Buffer: PBS.
Storage Conditions: -20℃.
Shipping Conditions: RT
