ME-180 Cell Line | CL-0155

ME-180 Cell Line | CL-0155 | Gentaur US, UK & Europe Disrtribition

Media: CM-0155

Organism: Homo sapiens, Human

Application: N/A

Background: Desmosomal attachments were observed between cells in monolayer culture and cytoplasmic tonofilaments were noted. Contamination with Mycoplasma arginini was detected and eliminated in 1970. Growth of ME-180 cells is inhibited by tumor necrosis factor alpha(TNF alpha). The cells contain human papillomavirus(HPV) DNA with greater homology to HPV-39 than HPV-18.

Age: 66 years

Tissue: Cervix, derived from metastatic site: omentum

Morphology: Epithelial

Growth Properties: Adherent

Doubling Time: N/A

Biosafty: 2

Medium: McCoy's 5A(PM150710)+10% FBS+1% P/S(PB180120)

Subculturing: Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.

Ratio: 1:2 to 1:4

Renewal: Every 2 to 3 days

Cryopreservation: Freeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase

Culture Conditions: Atmosphere: Air, 95%; CO2, 5% Temperature: 37℃

Effects: Yes, in nude mice forms well differentiated epidermoid carcinoma(grade I).

Duration: N/A

Lead Time: 3-5 days