MDCK [NBL-2] Cell Line | CL-0154

MDCK [NBL-2] Cell Line | CL-0154 | Gentaur US, UK & Europe Disrtribition

Media: CM-0154

Organism: Canis familiaris

Application: This cell line is a suitable transfection host and is useful for influenza research.

Background: This line is hyperdiploid and there is a bi-modal chromosome number distribution. There are no consistent identifiable marker chromosomes. One normal X chromosome is present in most spreads.The cells are positive for keratin by immunoperoxidase staining. MDCK cells have been used to study processing of beta amyloid precursor protein and sorting of its proteolytic products.

Age: Adult

Tissue: Kidney

Morphology: Epithelial

Growth Properties: Adherent

Doubling Time: N/A

Biosafty: 1

Medium: MEM(PM150467)+10% FBS+1% P/S(PB180120)

Subculturing: Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.

Ratio: 1:2 to 1:4

Renewal: Every 2 to 3 days

Cryopreservation: Freeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase

Culture Conditions: Atmosphere: Air, 95%; CO2, 5% Temperature: 37℃

Effects: N/A

Duration: N/A

Lead Time: 3-5 days