MDA-MB-436 Cell Line | CL-0383

MDA-MB-436 Cell Line | CL-0383 | Gentaur US, UK & Europe Disrtribition

Media: CM-0383

Organism: Homo sapiens, Human

Application: N/A

Background: The line is pleomorphic and most cells react intensely with anti tubulin antibody as demonstrated by indirect immunofluorescence staining.

Age: 43 years

Tissue: Mammary gland/breast, derived from metastatic site: pleural effusion

Morphology: Pleomorphic with multinucleated component cells

Growth Properties: Adherent

Doubling Time: N/A

Biosafty: 1

Medium: Leibovitz's L-15(PM151010)+10μg/ml Insulin+16μg/ml Glutathione+15% FBS+1% P/S(PB180120)

Subculturing: Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.

Ratio: 1:2 to 1:4

Renewal: Every 2 to 3 days

Cryopreservation: Freeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase

Culture Conditions: Atmosphere: Air, 95%; CO2, 5% Temperature: 37℃

Effects: No, in immunosuppressed mice; Yes, in semisolid medium.

Duration: N/A

Lead Time: 3-5 days