Description
MDA-kb2 Cell Line | CL-0704 | Gentaur US, UK & Europe Disrtribition
Media: CM-0704
Organism: Homo sapiens, Human
Application: N/A
Background: The MDA-kb2 cell line was derived from the breast cancer cell line, MDA-MB-453 (see ATCC HTB-131) by stable transfection with a mouse mammary tumor virus (MMTV) luciferase-neo reporter gene construct.
Age: 48 years
Tissue: Breast; Mammary gland
Morphology: Epithelial
Growth Properties: Adherent
Doubling Time: N/A
Biosafty: 1
Medium: Leibovitz's L-15(PM151010)++10%FBS+1%P/S(PB180120)
Subculturing: Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
Ratio: 1:2 to 1:4
Renewal: every 2 to 3 days
Cryopreservation: Freeze Medium: 55% Basal Medium+40% FBS+5% DMSOStorage temperature: Liquid nitrogen vapor phase
Culture Conditions: Atmosphere: Air, 95%; CO2, 5%Temperature: 37℃
Effects: N/A
Duration: N/A
Lead Time: 3-5 days
