Description
Magnetic Beads™ Streptavidin | SMB-B01-5mg| Gentaur Distribution US, UK & Europe
The Streptavidin-Magnetic Beads are 2.8 µm superparamagnetic particles covalently coupled to a highly pure form of streptavidin (SA) . The beads can be used to capture the biotinylated proteins or other molecules, because Streptavidin (SA) has an extraordinarily high affinity for biotin with a dissociation constant (Kd) on the order of 10−14 mol/L, the Biotinylated molecules can bind to the SA irreversibly. Streptavidin is a tetrameric protein purified from the bacterium Streptomyces avidin, and exhibits high binding affinity for biotin. Able to bind one molecule of biotin with each subunit. Streptavidin (PI=6.0-7.5) has lower level of non-specific binding to various biological components at physiological pH than avidin (PI=7.4), resulting from its isoelectric point (PI) . The Streptavidin-Magnetic Beads is easy to capture the biotinylated proteins or other molecules in Chemiluminescence procedures, and the bounded protein have no activity lost, this ready to use products could greatly save your protein coupling time and hassle, and help us get the best performance and highly reproducible results.
Source: The Streptavidin-Magnetic Beads are 2.8 µm superparamagnetic particles covalently coupled to a highly pure form of streptavidin (SA) . The beads can be used to capture the biotinylated proteins or other molecules, because Streptavidin (SA) has an extraordinarily high affinity for biotin with a dissociation constant (Kd) on the order of 10−14 mol/L, the Biotinylated molecules can bind to the SA irreversibly. Streptavidin is a tetrameric protein purified from the bacterium Streptomyces avidin, and exhibits high binding affinity for biotin. Able to bind one molecule of biotin with each subunit. Streptavidin (PI=6.0-7.5) has lower level of non-specific binding to various biological components at physiological pH than avidin (PI=7.4), resulting from its isoelectric point (PI) . The Streptavidin-Magnetic Beads is easy to capture the biotinylated proteins or other molecules in Chemiluminescence procedures, and the bounded protein have no activity lost, this ready to use products could greatly save your protein coupling time and hassle, and help us get the best performance and highly reproducible results.
Molecule: Streptavidin Beads.
Format: Powder.
Species: N/A.
Application: This product can be used as isotype control of protein pre-coupled magnetic beads（particle size 2μm） in various applications, and it also can be used for preparation of protein coupled magnetic beads by loading desired biotinylated proteins for immunocapture, biopanning and flow cytometry, etc. This product is produced non-sterile.
Kit Component: Application Method: a) Reconstitute the beads with ultrapure water to 1mg/mL, and wash the beads for three times. b) Dilute the biotinalytated protein or antibodies with Assay Buffer, and add to the beads, incubate for 60 minutes at room temperature in a rotary mixer. c) Wash the beads for four times, and add assay buffer to beads for next experiment.
Stability: This product is stable for 1 year when stored at -20 °C. Please avoid more than 3 freeze-thaw cycles. Immediate use after reconstitution is highly recommended.
Storage Conditions: -20℃, aviod light.
Shipping Conditions: RT
