LIGHT:HVEM [Biotinylated] Inhibitor Screening Assay Kit | 79684

LIGHT:HVEM [Biotinylated] Inhibitor Screening Assay Kit | 79684 | Gentaur US, UK & Europe Disrtribition

Category: Immunotherapy/Assay Kit

Application: This kit is useful for screening for inhibitors of HVEM binding to LIGHT.

Background: Herpesvirus entry mediator (HVEM, also known as tumor necrosis factor receptor superfamily member 14 (TNFRSF14) or CD270) and LIGHT (tumor necrosis factor superfamily member 14 (TNFSF14) or CD258) mediate costimulatory responses in T cell activation and proliferation during the immune response. The HVEM/LIGHT co-signaling pathway plays important roles in several biological processes, including autoimmunity, inflammation, bacterial and virus infection, transplant rejection and cancer.

Description: The LIGHT:HVEM[Biotinylated] Inhibitor Screening Assay Kit is designed for screening and profiling inhibitors of LIGHT:HVEM signaling. The key to this kit is the high sensitivity of detection of biotin-labeled HVEM by streptavidin-HRP. Only a few simple steps on a microtiter plate are required for the assay. First, LIGHT is coated on a 96-well plate. Next, HVEM is incubated with LIGHT on the plate. Finally, the plate is treated with streptavidin-HRP followed by addition of an HRP substrate to produce chemiluminescence, which can be measured using a chemiluminescence reader.

Product Type: Assay Kit

Shippement Condition: -80°C