Lec1 [Pro-5WgaRI3C] cell line | CL-0138

Lec1 [Pro-5WgaRI3C] cell line | CL-0138 | Gentaur US, UK & Europe Disrtribition

Media: CM-0138

Organism: Cricetulus griseus, Hamster

Application: N/A

Background: This line is a mutant clone selected for the resistance to wheat germ agglutinin. The cells are derived from the parental CHO clone Pro-5. The cells have N-linked carbohydrates with blocked Man5-GlcNAC2-Asn intermediate, which is due to the lack of GlcNAc glycosyl transferase. The line can potentially be used for the study of the influences of altered N-linked glycosylation to the cell function. It may also be used for compartmentalization of viral infection-induced or foreign DNA transfection-induced endogenous glycoproteins or glycoproteins.

Age: adult

Tissue: Ovary

Morphology: Epithelial

Growth Properties: Adherent

Doubling Time: N/A

Biosafty: 1

Medium: MEMα(PM150421)+10% FBS+1% P/S(PB180120)

Subculturing: Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.

Ratio: 1:2 to 1:4

Renewal: 2 to 3 times per week

Cryopreservation: Freeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase

Culture Conditions: Atmosphere: Air, 95%; CO2, 5% Temperature: 37℃

Effects: N/A

Duration: N/A

Lead Time: 3-5 days