LAG3:FGL1[Biotinylated] Inhibitor Screening Assay Kit | 79789

LAG3:FGL1[Biotinylated] Inhibitor Screening Assay Kit | 79789 | Gentaur US, UK & Europe Disrtribition

Category: Immunotherapy/Assay Kit

Application: This kit is useful for screening for inhibitors of LAG3 binding to FGL1.

Background: Lymphocyte-activation gene 3 (LAG3, also known as CD223) is a cell surface receptor that negatively regulates the activation and proliferation of T cells. Fibrinogen-like protein 1 (FGL1), a liver-secreted protein, is a functional LAG3 ligand. Blockade of the FGL1-LAG3 interaction is implicated in promoting antitumor immunity.

Description: The LAG3:FGL1[Biotinylated] Inhibitor Screening Assay Kit is designed for screening and profiling inhibitors of LAG3:FGL1. The key to this kit is the high sensitivity of detection of biotin-labeled FGL1 by streptavidin-HRP. Only a few simple steps on a microtiter plate are required for the assay. First, LAG3 is attached on a nickel-coated 96-well plate. Next, biotinylated FGL1 is incubated with LAG3 on the plate. Finally, the plate is treated with streptavidin-HRP followed by addition of an HRP substrate to produce chemiluminescence, which can be measured using a chemiluminescence reader.

Product Type: Assay Kit

Shippement Condition: -80°C