Description
KRT8 Antibody / Cytokeratin 8 | V3562-20UG | Gentaur US, UK & Europe Disrtribition
Family: Primary antibody
Formulation: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Format: Purified
Clone: KRT8/2174R
Host: Rabbit
Clonality: Recombinant Rabbit Monoclonal
Isotype: Rabbit IgG, kappa
Species Reactivity: Human
Application: IHC-P, WB
Application Details: Immunohistochemistry (FFPE): 0.5-1ug/ml for 30 min at RT,Western blot: 1-2ug/ml
Application Note: The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the recombinant Cytokeratin 8 antibody to be titered up or down for optimal performance.
1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Purity: Protein A affinity chromatography
Description: Cytokeratin 8 (CK8) belongs to the type II (or B or basic) subfamily of high molecular weight cytokeratins and exists in combination with cytokeratin 18 (CK18). Cytokeratin 8 is primarily found in the non-squamous epithelia and is present in majority of adenocarcinomas and ductal carcinomas. It is absent in squamous cell carcinomas. Hepatocellular carcinomas are defined by the use of antibody that recognizes only cytokeratin 8 and 18. Cytokeratin 8 exists on several types of normal and neoplastic epithelia, including many ductal and glandular epithelia such as colon, stomach, small intestine, trachea, and esophagus as well as in transitional epithelium.
Immunogen: A cytoskeletal preparation from human HeLa cells was used as the immunogen for this recombinant Cytokeratin 8 antibody.
Storage: Store the recombinant Cytokeratin 8 antibody at 2-8oC (with azide) or aliquot and store at -20 °C or colder (without azide).
Localization: Cytoplasmic
