Description
KRT10 Antibody / Cytokeratin 10 | V3603-20UG | Gentaur US, UK & Europe Disrtribition
Family: Primary antibody
Formulation: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Format: Purified
Clone: rKRT10/844
Host: Mouse
Clonality: Recombinant Mouse Monoclonal
Isotype: Mouse IgG1, kappa
Species Reactivity: Human
Application: IHC-P
Application Details: Immunohistochemistry (FFPE): 0.1-0.2ug/ml for 30 min at RT
Application Note: Optimal dilution of the recombinant KRT10 antibody should be determined by the researcher.
1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Purity: Protein G affinity chromatography
Description: This mAb recognizes a protein of 56.5kDa, identified as cytokeratin 10 (KRT10). KRT10 is expressed in all suprabasal layers of the epidermis. In the epidermis, expression of KRT10 strictly parallels the extent of differentiation; it is absent in the basal layer, appears in the first suprabasal layers and increases in concentration towards the granular layer. However, KRT10 is rarely detected in early stages of vulvar squamous carcinomas (tumors less than 2 cm, clinical stage I) regardless of the tumor grade. In larger and more advanced tumors (greater than 2 cm, clinical stages II and III), KRT10 is detected very frequently. Expression is related to maturation of malignant keratinocytes, being preferentially detected in more-differentiated parts.
Immunogen: Recombinant human Cytokeratin 10 protein was used as the immunogen for the recombinant KRT10 antibody.
Storage: Store the recombinant KRT10 antibody at 2-8oC (with azide) or aliquot and store at -20 °C or colder (without azide).
Localization: Cytoplasmic
