KRAS (G12D), Isoform A, His-Tag, GppNHp-Loaded Recombinant | 101481

KRAS (G12D), Isoform A, His-Tag, GppNHp-Loaded Recombinant | 101481 | Gentaur US, UK & Europe Disrtribition

Category: RAS Signaling/Protein

Application: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Background: N/A

Description: Recombinant human KRAS (KRAS proto-oncogene GTPase), isoform a, encompassing amino acids 2-186(end). This construct contains an N-terminal His-tag (6xHis) followed by an optimal TEV protease target sequence that allows cleavage of the His-tag from the protein (TEV: tobacco etch virus cysteine protease, BPS Bioscience #50308). The protein also contains mutation of interest G12D. The protein was affinity purified and loaded with GppNHp, a non-hydrolyzable GTP analog. Unbound GppNHp was removed by spin column. Ready for use in KRAS-RAF binding studies or inhibitor assays.

Product Type: Protein

Shippement Condition: -80°C