Description
KLE cell line | CL-0133 | Gentaur US, UK & Europe Disrtribition
Media: CM-0133
Organism: Homo sapiens, Human
Application: N/A
Background: The KLE cell line was established by G. R. Richardson in 1984 from endometrial tumor tissue in a 64-year-old female Caucasian. The chromosome of KLE cells is aneuploid, with multiple chromosome numbers in the range of 51-66. Electron microscopy of tumors formed in nude mice shows microvilli and junctional complexes, and nucleolar channel systems are present that are similar to those seen in normal endometrium under progestational stimulation.
Age: female, 64 years
Tissue: Uterus, Endometrium
Morphology: Epithelial
Growth Properties: Adherent
Doubling Time: ~114 hours
Biosafty: 1
Medium: DMEM/F12(PM150312)+10% FBS+1% P/S(PB180120)
Subculturing: Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
Ratio: 1:2 to 1:4
Renewal: 2 times per week
Cryopreservation: Freeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase
Culture Conditions: Atmosphere: Air, 95%; CO2, 5% Temperature: 37℃
Effects: N/A
Duration: N/A
Lead Time: 3-5 days
