KLE cell line | CL-0133

KLE cell line | CL-0133 | Gentaur US, UK & Europe Disrtribition

Media: CM-0133

Organism: Homo sapiens, Human

Application: N/A

Background: The KLE cell line was established by G. R. Richardson in 1984 from endometrial tumor tissue in a 64-year-old female Caucasian. The chromosome of KLE cells is aneuploid, with multiple chromosome numbers in the range of 51-66. Electron microscopy of tumors formed in nude mice shows microvilli and junctional complexes, and nucleolar channel systems are present that are similar to those seen in normal endometrium under progestational stimulation.

Age: female, 64 years

Tissue: Uterus, Endometrium

Morphology: Epithelial

Growth Properties: Adherent

Doubling Time: ~114 hours

Biosafty: 1

Medium: DMEM/F12(PM150312)+10% FBS+1% P/S(PB180120)

Subculturing: Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.

Ratio: 1:2 to 1:4

Renewal: 2 times per week

Cryopreservation: Freeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase

Culture Conditions: Atmosphere: Air, 95%; CO2, 5% Temperature: 37℃

Effects: N/A

Duration: N/A

Lead Time: 3-5 days