KATO III [KATO 3] Cell Line | CL-0372

KATO III [KATO 3] Cell Line | CL-0372 | Gentaur US, UK & Europe Disrtribition

Media: CM-0372

Organism: Homo sapiens, Human

Application: N/A

Background: N/A

Age: 55 years adult

Tissue: Stomach, derived from metastatic site: pleural effusion and supraclavicular and axillary lymph nodes and douglas cul-de-sac

Morphology: Spherical

Growth Properties: Mixed, Adherent And Suspension

Doubling Time: N/A

Biosafty: 1

Medium: IMDM(PM150510)+10% FBS+1% P/S(PB180120)

Subculturing: Remove and discard culture medium. These cells grow as a mixture of floating and adherent cells. Sometimes many cells are floating, they can be harvested by centrifugation of medium instead of discarding it. Add 1.0 to 2.0 mL of 0.25% (w/v) Trypsin-0.53mM EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C.

Ratio: 1:2 to 1:4

Renewal: Every 2 to 3 days

Cryopreservation: Freeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase

Culture Conditions: Atmosphere: Air, 95%; CO2, 5% Temperature: 37℃

Effects: Yes, in cheek pouches of anti thymocyte serum treated hamsters. No, in nude mice.

Duration: N/A

Lead Time: 3-5 days