JeKo-1 Cell Line | CL-0128

JeKo-1 Cell Line | CL-0128 | Gentaur US, UK & Europe Disrtribition

Media: CM-0128

Organism: Homo sapiens, Human

Application: JeKo-1 cells are highly tumorigenic in SCID mice.

Background: The cells overexpress cyclin D1, Bcl-2, c-Myc and Rb proteins. Bcl-1/J(H) gene rearrangement was confirmed by polymerase chain reaction. JeKo-1 cells are highly tumorigenic in SCID mice. Ref JeKo-1 cells are negative for Epstein-Barr virus and express IgM, a B-cell phenotype.

Age: 78 years

Tissue: Peripheral blood, mantle cell lymphoma

Morphology: Lymphoblast

Growth Properties: Suspension

Doubling Time: N/A

Biosafty: 1

Medium: RPMI-1640(PM150110)+20% FBS+1% P/S(PB180120)

Subculturing: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2×10^5 viable cells/ml. Maintain cell density between 1×10^5 and 1×10^6 viable cells/ml.

Ratio: 1:2 to 1:4

Renewal: Every 2 to 3 days

Cryopreservation: Freeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase

Culture Conditions: Atmosphere: Air, 95%; CO2, 5% Temperature: 37℃

Effects: N/A

Duration: N/A

Lead Time: 3-5 days