Description
JeKo-1 Cell Line | CL-0128 | Gentaur US, UK & Europe Disrtribition
Media: CM-0128
Organism: Homo sapiens, Human
Application: JeKo-1 cells are highly tumorigenic in SCID mice.
Background: The cells overexpress cyclin D1, Bcl-2, c-Myc and Rb proteins. Bcl-1/J(H) gene rearrangement was confirmed by polymerase chain reaction. JeKo-1 cells are highly tumorigenic in SCID mice. Ref JeKo-1 cells are negative for Epstein-Barr virus and express IgM, a B-cell phenotype.
Age: 78 years
Tissue: Peripheral blood, mantle cell lymphoma
Morphology: Lymphoblast
Growth Properties: Suspension
Doubling Time: N/A
Biosafty: 1
Medium: RPMI-1640(PM150110)+20% FBS+1% P/S(PB180120)
Subculturing: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2×10^5 viable cells/ml. Maintain cell density between 1×10^5 and 1×10^6 viable cells/ml.
Ratio: 1:2 to 1:4
Renewal: Every 2 to 3 days
Cryopreservation: Freeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase
Culture Conditions: Atmosphere: Air, 95%; CO2, 5% Temperature: 37℃
Effects: N/A
Duration: N/A
Lead Time: 3-5 days
