JARID1A (KDM5A) Chemiluminescent Assay Kit | 50513

JARID1A (KDM5A) Chemiluminescent Assay Kit | 50513 | Gentaur US, UK & Europe Disrtribition

Category: Demethylase/Assay Kit

Application: Great for studying enzyme kinetics and HTS applications.

Background: JARID1A is a member of the Jumonji, AT-rich interactive domain 1 (JARID1) histone demethylase protein family. It binds directly, with several other proteins, to retinoblastoma protein which regulates cell proliferation.

Description: The JARID1A Chemiluminescent Assay Kit is designed to measure JARID1A activity for screening and profiling applications. The key to the JARID1A Chemiluminescent Assay Kit is a highly specific antibody that recognizes demethylated substrate. With this kit, only three simple steps on a microtiter plate are required for detection of demethylase activity. First, JARID1A enzyme is incubated with the methylated H3 peptide for one hour. Next, primary antibody is added. Finally, the plate is treated with an HRP-labeled secondary antibody followed by the addition of the HRP substrate to produce chemiluminescence that can be measured using a chemiluminescence reader.

Product Type: Assay Kit

Shippement Condition: -80°C (dry ice)