Description
Involucrin Antibody | V2164-20UG | Gentaur US, UK & Europe Disrtribition
Family: Primary antibody
Formulation: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Format: Purified
Clone: SY5
Host Animal: Mouse
Clonality: Monoclonal (mouse origin)
Species Reactivity: Human
Application: IHC-P
Buffer: N/A
Limitation: This Involucrin antibody is available for research use only.
Purity: Protein G affinity chromatography
Description: This antibody recognizes a protein of 66-170 kDa, identified as involucrin. In Western blotting of cultured human keratinocytes, this antibody reacts with a 120 kDa protein. It stains the involucrin in a variety of sizes: 170 kDa in MCF-7 cells, a doublet of ~115 and 150kDa in gorilla and owl monkey, 66kDa in dog, and a doublet of 105kDa in pig. Its epitope maps between amino acids 421-568 of human involucrin. Involucrin is expressed in a range of stratified squamous epithelia, including the cornea, which lacks a distinct cornified layer. In normal epidermis, it is first expressed in the upper spinous layers, and in keratinocyte cultures, all cells that have left the basal layer express it. Involucrin expression is altered in pathological conditions: in psoriasis and other benign epidermal hyperplasias, involucrin expression begins closer to the basal layer than normal; expression is abnormal in squamous cell carcinomas and premalignant lesions, and is reduced in severe dysplasias of the larynx and cervix.
Immunogen: Purified Involucrin from human keratinocytes was used as the immunogen for this antibody.
Storage: Store the Involucrin antibody at 2-8oC (with azide) or aliquot and store at -20 °C or colder (without azide).
